CAMK2A antibody (pThr286)

Catalog No. ABIN361458

This anti-CAMK2A antibody is a Rabbit Polyclonal antibody detecting CAMK2A in WB, IHC and ELISA. Suitable for Rat. This Primary Antibody has been cited in 11+ publications.

Quick Overview for CAMK2A antibody (pThr286) (ABIN361458)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr286

Specificity: Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: human, Xenopus

Characteristics: Our CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-CAMK2A antibody (ABIN361458)

Lee, Shin, Zhong, Kim, Kim, Kim, Heo, Kim, Yeo, Kim, Liu: "Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes." in: Nature communications, Vol. 9, Issue 1, pp. 3434, (2018) (PubMed).

Murakoshi, Shin, Parra-Bueno, Szatmari, Shibata, Yasuda: "Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor." in: Neuron, Vol. 94, Issue 1, pp. 37-47.e5, (2017) (PubMed).

Bernard, Castano, Bayer, Benke: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." in: Neuropharmacology, Vol. 84, pp. 1-12, (2014) (PubMed).

Boguslavsky, Chiu, Foley, Osorio-Fuentealba, Antonescu, Bayer, Bilan, Klip: "Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles." in: Molecular biology of the cell, Vol. 23, Issue 20, pp. 4065-78, (2012) (PubMed).

Coultrap, Barcomb, Bayer: "A significant but rather mild contribution of T286 autophosphorylation to Ca2+/CaM-stimulated CaMKII activity." in: PLoS ONE, Vol. 7, Issue 5, pp. e37176, (2012) (PubMed).

Kothmann, Trexler, Whitaker, Li, Massey, OBrien: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, (2012) (PubMed).

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).

Ferrario, Loweth, Milovanovic, Ford, Galiñanes, Heng, Tseng, Wolf: "Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²?-permeable AMPA receptors during the incubation of cocaine craving." in: Neuropharmacology, Vol. 61, Issue 7, pp. 1141-51, (2011) (PubMed).

Coultrap, Buard, Kulbe, DellAcqua, Bayer: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, (2010) (PubMed).

Vest, OLeary, Coultrap, Kindy, Bayer: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, (2010) (PubMed).

Townsend, Mehta, Selkoe: "Soluble Abeta inhibits specific signal transduction cascades common to the insulin receptor pathway." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33305-12, (2007) (PubMed).

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CaM Kinase II

Background:

Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Description Logic: Our CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody is produced . It detects CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: CAMK2A/B

Molecular Weight: 50 kDa/60 kDa

Gene ID: 25400

UniProt: P11275

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction